Lorant Szekvolgyi
Research project
Exploring the SET1 hisonte methylase complex in meiosis: from gene loops to DNA double-strand break (DSB) formation
Project summary
Initiation of meiotic recombination is impacted by histone H3 methyation that is carried out by the Set1 complex (COMPASS) in budding yeast. Tagging meiotic DSB sites with the broadly localized H3K4me3 mark is a molecular strategy that provides flexibility to ensure transmission of a large diversity of recombinant haplotypes to the offspring. In the current application we aim to find a causative relationship between the presence of the COMPASS subsunit Spp, meiotic DSBs and gene-loop formation upon initiation of meiotic recombination.
The current Iméra/INSERM project will be carried out in collaboration with the laboratory of Dr. Vincent Géli. In the planned “biophysical genomics” experiments we will target various histone modifying enzymes and recombinosome proteins to predefined chromosomal loci to induce meiotic recombination in the yeastS cerevisiae. We expect that our results will open new horizons to drill down to the genes to unravel the genetic basis of 3D genome architecture in the context of meiotic recombination.
